CREATING A "PIXELS TO MICRONS" CONVERSION TABLE   Revised Dec. 2025

The simplest way to create a "pixels to microns" conversion table, for a specific digital camera, is to borrow or buy a special microscope slide that has a one millimeter ruler engraved in it. They can be bought through the internet (it's priced between $13 and $19).
Here is an example image.
 


Then, you need to capture images of this ruler/slide with your digital camera, using the available camera resolutions and using each of the objective lenses (e: 4x, 10x, 40x,100x). Then move the captured images to a folder. Then, using an image manipulation application, measure (in pixels) a part of the ruler. This will give you the numbers necessary to compute the conversion factor that will be entered in the table. I am including bellow, some samples, so you can see the simple math applied to each image. I should mention that your camera should have a 10x microscope lense. The microscope camera lense, that I have, can be used with a Nikon or a Canon camera, and it is a 10x, and it slides into any of the microscope visual ports.













If you create a table for your camera, and would like to see it posted on this web site, I will be glad to add it. And if you would like your name posted with the table, that's OK too. Just send me email, with the table, to iq@manyportals.info

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